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The final PEG pellets were re-suspended in PBS at 1:10 homogenization volume, and final protein concentration was measured using bicinchoninic acid (BCA) kit

Hassinen et al

This addiction primarily stems from the presence of nicotine, a potent psychoactive substance found in tobacco, according to an article by the Center of Addiction and Mental Health titled Nicotine Dependence. The addictive nature of tobacco leads individuals to develop a tolerance, necessitating higher amounts to achieve the same effects, ultimately reinforcing the cycle of dependence